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. 2016 Sep 1;6:32087. doi: 10.1038/srep32087

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Copyright © 2016, The Author(s)

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(A) Western blot analysis of cleaved caspase 3 and cleaved PARP. (B) Flow cytometric analysis of apoptosis after Z-VAD-FMK treatment (**P < 0.01). (C) Immunofluorescence of F-actin in PAN treated mouse podocytes after Z-VAD-FMK treatment (PAN: 50 μg/ml; Z-VAD-FMK: 10 μM). Scale bar, 100 μm; (D) Transwell migration assays of podocyte motility after Z-VAD-FMK treatment (**P < 0.01; original magnification, X100). All experiments were performed for 3 times, and the results were shown as mean ± SD.