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. 2016 Sep 1;6:32087. doi: 10.1038/srep32087

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Copyright © 2016, The Author(s)

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(A) Fluorescent staining of podocyte mitochondria with Mito Tracker Red CMXRos after CsA and FK506 treatment for 24 h in PAN-treated podocytes, the expression of cytochrome c was also detected. (B) Quantification also indicated increased expression of cytochrome c (cytosolic fraction) was inhibited by CsA and FK506. (C) Flow cytometric analysis and fluorescent staining (D) of the effects of CsA and FK506 pretreatment on PAN-induced MMP loss in podocytes (*P < 0.05). (E) Flow cytometric analysis of the effects of CsA and FK506 pretreatment on PAN-induced ATP reduction in podocytes (*P < 0.05). All experiments were performed for 3 times, and the results were shown as mean ± SD.