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. 2016 Sep 1;6:32538. doi: 10.1038/srep32538

Figure 2. PCV2 inhibits SOCS3 expression and NF-κB signaling induced by TNF-α and IL-6 in PBMCs from PCV2-infected piglets.

Figure 2

PBMCs separated from (A) healthy (n = 5) and (B) PCV2-infected piglets (n = 5) were treated with TNF-α (50 ng/ml) or IL-6 (100 ng/ml) for 0, 30, 45, 60, and 120 min. At each time point, PBMCs were lysed, and SOCS3 expression was analyzed by immunoblotting. PBMCs from (C) healthy (n = 5) and (D) PCV2-infected piglets (n = 5 of 11) were treated with TNF-α or IL-6 for 0 and 30 min. IκB-α protein levels were measured by immunoblotting. The intensities of the bands in the protein lysates were normalized to that of β-actin. Statistical data were analyzed by one-way analysis of variance (*P < 0.05; **P < 0.01; and ***P < 0.001; ns, not significant (P > 0.05)). All data are expressed as the mean ± SD. The samples were derived from the same experiment, and the blots of actin and IκB-α were processed in parallel. The blots of SOCS3 were performed in another gel using the same concentrations of the same samples. The two gels were run under the same conditions.