Figure 3. The effect of ccpA deletion on the GAS transcriptome.

(a) Linear representation of the genome of GAS serotype strains showing the effect of ccpA-inactivation on gene expression depicted as log fold change. (b) A weighted Venn diagram displaying the distribution and overlap of the CcpA-affected genes in all three serotypes was generated using the BioVenn application⁶⁰. Arrows indicate the number of genes repressed (down) and activated (up) by CcpA in individual serotypes and in the core regulon. (c) Genes affected by CcpA in each serotype are shown by order of COG categories. Each colored circle denotes the enrichment of a specific COG in the serotype strain. [C] Energy production and conversion; [E] Amino acid transport and metabolism; [G] Carbohydrate transport and metabolism; [I] Lipid transport and metabolism; [J] Translation, ribosomal structure and biogenesis; [L] Replication, recombination and repair; [M] Cell wall/membrane/envelope biogenesis; [N] Cell motility; [O] Posttranslational modification, protein turnover, chaperones; [R] General function prediction only; [S] Function unknown; [U] Intracellular trafficking, secretion, and vesicular transport.