Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 1;6:32443. doi: 10.1038/srep32443

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) KaiC was incubated in the presence of ATP at 30 °C for 24 h, and aliquots were subjected to SDS-PAGE (upper panel) and BN-PAGE (lower panel) analyses. (B) Elution profiles of KaiC at 0, 12 and 24 h were generated from gel filtration chromatography experiments. Arrowheads at the top of the panel indicate elution volumes of molecular mass standards. (C) KaiC was incubated in the presence of ATP at 30 °C, and aliquots were subjected to CBB-free native-PAGE at 0, 12 and 24 h with or without 0.25% CBB. (D) KaiC was incubated in the presence of ATP at 30 °C for 12 h, and was subjected to CBB-free native-PAGE following addition of CBB (0%, 0.25%, 0.5% and 1%).