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. 2016 Sep 1;6:32443. doi: 10.1038/srep32443

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) ATPase activity of E77Q/E78Q-KaiC relative to that of wild-type (WT) KaiC. The data represent the means ± SD from three independent experiments. (B) E77Q/E78Q-KaiC was incubated in the presence or absence of KaiA at 30 °C for 24 h, and was then subjected to SDS-PAGE (upper panel) and BN-PAGE (lower panel) analyses.