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. 2016 Jun 23;35(17):1853–1867. doi: 10.15252/embj.201593148

Figure 7. Joubert syndrome‐associated INPP5E mutations compromise autophagy.

Figure 7

  1. Joubert syndrome‐associated INPP5E mutations (Bielas et al, 2009) used in this study.
  2. N1E‐115 cells stably expressing the indicated mutants treated with siControl or siINPP5E were cultured in growth medium with or without 125 nM Baf.A1 for 2 h and analyzed by immunoblot using anti‐LC3 and anti‐GAPDH antibodies. The mutants were resistant to mouse siINPP5E because target sequence of the siRNA is different from the human correspondent sequence.
  3. Quantitation of protein signal intensities from immunoblots in (B) showing differences in LC3‐II levels in the presence and absence of Baf.A1 following normalization to the control protein GAPDH. Results represent means ± s.d. of three independent experiments. **P < 0.01; *P < 0.05.

Source data are available online for this figure.