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. 2016 Jul 27;17(9):1304–1313. doi: 10.15252/embr.201541476

Figure EV2. Expression validation of mESC‐specific SATS isoforms by reverse transcription PCR (RT–PCR).

Figure EV2

  1. The heat map for the read coverage of the 5′ specific region of the SATS (top) and common (bottom) isoforms in three mESC lines and NSC, TTF, and ADSC. Read coverage abundance of the 5′ specific region of each isoform is shown as log2 based transformation of FPKM values.
  2. Expression validation of randomly selected SATS isoforms in mESCs and somatic cells by RT–PCR, extension of Fig 1A. NC represents PCR negative control without template. Numbers in parenthesis represent the abundance rank of SATS isoforms.
  3. Expression validation of the top 18 highly abundant mESC‐specific SATS isoforms and their corresponding common isoforms by RT–PCR. “_C” and “_S” denote the common and SATS isoform of each gene, respectively. NC represents PCR negative control without template. Numbers in parenthesis represent the abundance rank of SATS isoforms.

Source data are available online for this figure.