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. 2015 Apr 9;24(13):3764–3774. doi: 10.1093/hmg/ddv120

Figure 3.

Figure 3.

Generation of live monkeys carrying mutations in the DMD gene. (A) CRISPR/Cas9-mediated targeting in monkeys. (B) Identified sequence mutations in full-term stillborn monkeys. (C) A photo of two monkeys at the age of 76 days, which were found to carry Cas9-mediated mutations. (D) Representative PCR analysis of mutations in exon 4 DMD gene of the DMD gene in full-term stillborn monkeys Mut-1 and Mut-2 and live monkeys (Mut-3, -4, -5 and -6). T7EN1 and enzymatic digestion were performed. WT, wild type; PC, positive control. (E) Identified sequence mutations in live monkeys.