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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biochemistry. 2016 Jul 1;55(27):3727–3734. doi: 10.1021/acs.biochem.6b00480

Figure 2.

Figure 2

Effects of I-CBP112 on full-length p300-mediated nucleosome acetylation. (A) Autoradiograph image of reaction mixtures containing 10 nM p300, 0.5 μM nucleosome, 5 μM [14C]acetyl-CoA and varying concentrations of I-CBP112. (B) Quantification of duplicate reactions in panel A. EC50 obtained from a standard binding isotherm (reported average ± span of duplicates). (C) p300-catalyzed nucleosome acetylation time course with or without I-CBP112 (40 μM) [average ± span (duplicates)]. Comparison of fits with or without I-CBP112 shows p = 0.005 for the linear initial 5 min. (D) p300-catalyzed nucleosome acetylation with variable nucleosome concentration with or without I-CBP112 (40 μM). (E) Quantification of duplicate reactions as seen in panel D [points = average ± SEM (n = 2–3)]. Comparison of fits showed the curves were significantly different (p < 0.0001).