Effects of CBP30 on full-length p300-mediated nucleosome acetylation. (A) Quantification of acetyltransferase reaction mixtures containing 10 nM p300, 0.5 μM nucleosomes, 5 μM [14C]acetyl-CoA, and varying concentrations of CBP30 [average ± span (duplicates)]. (B) Autoradiograph image of reaction mixtures containing 10 nM p300, 0.5 μM nucleosome, 5 μM [14C]acetyl-CoA, and designated I-CBP112 and CBP30 concentrations. (C) Quantification of the gel in panel B. Error bars represent the span of data (n = 2). (**p < 0.01; *p < 0.05 by one-way ANOVA with Dunnet correction for a normalized control; for all others, p > 0.35).