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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biochemistry. 2016 Jul 1;55(27):3727–3734. doi: 10.1021/acs.biochem.6b00480

Figure 6.

Figure 6

Effects of I-CBP112 on histone H3K18 acetylation and proliferation in cancer cells. (A) Western blots of H3K18 acetylation and total H3 levels upon treatment of LNCaP cell lines with I-CBP112 (4 h). Representative blots of duplicate experiments. Fold change relative to no ligand of the ratios of H3K18ac to total H3 [average ± span (n = 2)]. (B) Same as panel A but in KG1a cells treated for 6 h. (C) Effects of JQ1 on H3K18ac in LNCaP cells (duplicates). All bands from the same gel. (D) I-CBP112 shows an IC50 of 5.5 ± 1.1 μM. The Hill slope = 1.3 ± 0.1 on LNCaP cell proliferation after 72 h ([3H]thymidine incorporation) (n = 3). (E) Same as panel D, but KG1a cells show an IC50 of 9.1 ± 1.2 μM and a Hill slope of 0.82 ± 0.09 (n = 4).