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. 2016 Jul 7;8(3):232–243. doi: 10.1093/jmcb/mjw002

Figure 2.

Figure 2

Immunostaining of YY1 and pAKT in breast cancer tissues and cells. (A) Examples of YY1 and pAKT(S473) staining in breast cancer tissue samples. Top and middle panels are two samples with cytoplasmic YY1 staining, while the bottom panel is a sample with nuclear YY1 staining. (B–D) Immunostaining of YY1, pAKT(S473), pAKT(T308), and total AKT in MDA-MB-231 cells without and with Dox-induced YY1 knockdown. (E) Immunostaining without primary antibodies as background control. (F) The orientation of the antibodies used. DIC, differential interference contrast.