Figure 6.

YY1 OPB domain is necessary for breast cancer cell proliferation and tumor formation. MDA-MB-231 cells were infected by pSL5/HA-YY1 wt or ΔOPB, with endogenous YY1 simultaneously silenced by shYY1-2, and tested by WST-1 assay (A) and xenograft tumor formation experiment (B–D). (A) Cell proliferation data were derived from samples in triplicates for three times. Bottom panel, MDA-MB-231 cells for WST-1 assays were analyzed by western blot. (B–D) Xenograft tumor volume (B), weight (C), and image (D) are presented. Bottom panel, representative immunohistochemical analyses of xenograft tumors by using YY1, pAKT(S473), and Ki-67 antibodies.