Figure 5.

TDP and dFMRP associate with actively translating polysomes to regulate the translation of futsch. (A) Distribution of TBPH, S6 ribosomal protein, and dFMRP, throughout polysome gradients from w¹¹¹⁸ controls. (B and C) Distribution of TDP-43, ribosomal protein S6, and dFMRP throughout polysome gradients in the context of TDP-43 WT (B) or G298S (C). (D) Western blot for Futsch protein levels. Antibodies as indicated on left. Actin was used as a loading control. Genotypes as indicated on bottom. (E) Quantification of Futsch protein levels normalized to Actin. (F–H) qPCR for futsch mRNA distribution in polysome fractionation input (F), RNP fractions (G), and polysome fractions (H).