Figure 6.

Futsch dependent neuromuscular junction alterations are restored by dFMRP OE. (A–F) Larval neuromuscular junctions of D42>w¹¹¹⁸ controls (A and B) and larvae expressing TDP-43^WT (C and D) or TDP-43^G298S (E and F) in motor neurons, immunostained for the neuronal membrane marker HRP (A, C and E) and Futsch (B, D and F). Arrow heads indicate satellite boutons. Asterisks indicate Futsch loops. (G–L) Larval neuromuscular junctions of dFMRP OE in a TDP-43^WT (I and J) or TDP-43^G298S (K and L) background compared to dFMRP OE controls (G and H), immunostained the neuronal membrane marker HRP (G, I and K) and Futsch (H, J and L). Arrowheads indicate satellite boutons. Asterisks indicate Futsch loops. (M–O) Quantification of Futsch Intensity (M), satellite boutons (N), and Futsch loops (O). Scale bar (A) 5 μm.