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. 2016 Aug 12;48(8):e252. doi: 10.1038/emm.2016.68

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Copyright © 2016 KSBMB.

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Effect of S650 mutation on protein interactions and H3/H4 histone binding. (a) Purified recombinant Flag-HIRA (WT, S650A and S650D) and GST-Asf1a were incubated in vitro and precipitated using an anti-HIRA antibody (WC15). IP pellets were probed with anti-Asf1a and anti-HIRA (WC119) antibodies. (b, c) HA-HIRA (wild type, S650A, or S650D) was expressed in 293T cells. HA-HIRA was subjected to IP using an anti-HA antibody and the endogenous proteins interacting with HIRA were detected by immunoblotting with the corresponding specific antibodies. WT, wild type.