Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 12;48(8):e252. doi: 10.1038/emm.2016.68

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

PMC Copyright notice

Phosphorylation mimicking form of HIRA prevents the incorporation of H3.3 and the activation of muscle genes. (a) C2C12 cells stably expressing empty vectors or each type of HA-hHIRA were generated and confirmed by IP. Cell extracts were subjected to IP using an anti-HA antibody and probed with an anti-WC119 HIRA antibody that recognized both human and mouse HIRA proteins. (b) C2C12 cells were treated with siRNAs specifically targeting endogenous mouse HIRA. The mHIRA knockdown efficiency was monitored by qRT-PCR. (c) C2C12 cell lines were treated with mHIRA-targeting siRNAs, induced to differentiate for 3 days, and subjected to qRT-PCR for analysis of mRNA levels. (d) H3.3 ChIP was performed to monitor H3.3 incorporation in the MyoD regulatory regions in the indicated C2C12 cell lines. Chromatin solutions prepared from myotubes were subjected to IP using an anti-H3.3 antibody. Error bars represent s.d., n=2. IP, immunoprecipitation; mRNA, messenger RNA; qRT-PCR, quantitative reverse transcription PCR; siRNA, small interfering RNA.