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. 2016 Aug 26;48(8):e255. doi: 10.1038/emm.2016.73

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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HMGA1 is highly expressed in spheroids from A2780 ovarian cancer cells. (a) RT-PCR analysis and western blotting analysis of A2780 adherent cells and spheroid cells are shown in the upper panels with HMGA1 probes along with GAPDH probes. Quantification of HMGA expression by western blotting after normalization using GAPDH expression is shown in the lower panel. *P<0.05. (b, c) Flow cytometric analysis and sorting of A2780 spheroid cells after incubation with an ALDH substrate is shown. Western blotting analysis of whole spheroid cells, ALDH⁻ cells and ALDH⁺ cells after sorting is shown with the indicated antibodies.