Figure 3. Physapubescin selectively induces apoptosis in VHL-null 786-O cells but not VHL wild-type Caki-2 cells.

(A) the upper panel: live cell morphology under phase-contrast light microscope (Magnification: X100). DAPI staining of nuclear morphology under fluorescence microscope (Magnification: X200). Representative pictures were shown from a random field. (B) cells with nuclear condensation and fragmentation were counted as apoptotic cells in 12 fields in each group. The percentage of apoptotic cells was calculated and presented as mean ± SEM. (C,D) cells were stained by Annexin V and PI and analyzed by flow cytometry. The data are presented by bar figure and mean ± SEM of three independent experiments. “*” and “**” denote “P < 0.05” and “P < 0.01” respectively.