Table 2.

Mapped contig results of RNA-Seq reads from null segregant (NG) and OsFAD2-1RNAi rice lines at three grain developmental stages

Contigs	Null segregant			Os-FAD2-1 RNAi
	Sample1	Sample 2	Sample 3	Sample 1	Sample 2	Sample 3
10 DAA
Counted contigs	1,474,350	2,451,049	912,841	2,305,750	7,974,195	3,179,294
Unique contigs	1,403,705	2,334,799	858,280	2,090,665	7,406,550	3,050,886
Non-S contigs	70,645	116,250	54,561	215,085	567,645	128,408
Un-C contigs	380,469	691,998	678,922	221,969	1,119,121	590,977
Total contigs	1,854,819	3,143,047	1,591,763	2,527,719	9,093,316	3,770,271
Counted contigs (%)	79.49	77.98	57.35	91.22	87.69	84.33
Unique contigs (%)	75.68	74.28	53.92	82.71	81.45	80.92
Non-S contigs (%)	3.81	3.70	3.43	8.51	6.24	3.41
Un-C contigs (%)	20.51	22.02	42.65	8.78	12.31	15.67
15 DAA
Counted contigs	1,721,045	4,038,637	6,507,485	1,260,698	4,102,787	5,184,375
Unique contigs	1,580,034	3,759,033	5,944,747	1,211,621	3,877,771	4,889,384
Non-S contigs	141,011	279,604	562,738	49,077	225,016	294,991
Un-C contigs	210,716	347,568	496,403	385,614	1,123,069	436,145
Total contigs	1,931,761	4,386,205	7,003,888	1,646,312	5,225,856	5,620,520
Counted contigs (%)	89.09	92.08	92.91	76.58	78.51	92.24
Unique contigs (%)	81.79	85.70	84.88	73.60	74.20	86.99
Non-S contigs (%)	7.30	6.37	8.03	2.98	4.31	5.25
Un-C contigs (%)	10.91	7.92	7.09	23.42	21.49	7.76
20 DAA
Counted contigs	2,945,375	1,943,916	1,348,074	3,914,475	791,645	3,627,328
Unique contigs	2,797,599	1,778,024	1,212,290	3,446,816	734,727	3,386,969
Non-S contigs	147,776	165,892	135,784	467,659	56,918	240,359
Un-C contigs	447,772	250,284	168,761	441,185	464,829	492,027
Total contigs	3,393,097	2,194,200	1,516,835	4,355,660	1,256,474	4,119,355
Counted contigs (%)	89.09	88.59	88.87	89.87	63.01	88.06
Unique contigs (%)	86.80	81.03	79.92	79.13	58.48	82.22
Non-S contigs (%)	82.45	7.56	8.95	10.74	4.53	5.83
Un-C contigs (%)	4.36	11.41	11.13	10.13	36.99	11.94

Non-S contigs- Non-specific contigs; Un C contigs-Un-counted contigs