Table 1.
Percentage of Each Monocyte Subset that Produces Different Intracellular Cytokines Ex Vivo and In Vitro
| Elderly Group (n = 20) | Young Group (n = 20) | p Values | p Values | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Ex Vivo | In Vitro | Ex Vivo | In Vitro | Ex Vivo | In Vitro | ||||
| LMMS | LPS | LMMS | LPS | LMMS | LPS | ||||
| Classical monocytes | |||||||||
| IL1-α | 0.36 (0.07–1.65) | 0.70 (0.09–3.42) | 7.35 (0.37–21.26) | 0.04 (0.02–0.11) | 0.09 (0.02–0.50) | 1.42 (0.62–6.59) | .001** | .060*** | .200 |
| IL1-β | 0.39 (0.19–1.45) | 8.00 (3.13–15.49) | 35.45 (18.89–54.20) | 0.13 (0.06–0.29) | 3.12 (1.26–5.82) | 13.45 (4.33–34.88) | .001** | .003** | .068*** |
| IL-6 | 0.59 (0.31–1.17) | 4.16 (2.50–8.97) | 23.35 (10.54–39.28) | 0.25 (0.10–0.47) | 3.05 (1.58–6.73) | 14.15 (4.47–34.53) | .002** | .140 | .240 |
| IL-8 | 2.26 (1.41–4.13) | 15.30 (8.19–27.94) | 44.19 (19.07–62.32) | 1.15 (0.49–1.62) | 12.63 (4.55–18.90) | 26.18 (11.85–58.81) | .007** | .080*** | .200 |
| IL-10 | 0.11 (0.04–0.50) | 0.03 (0.00–0.20) | 0.01 (0.00–1.33) | 0.18 (0.04–0.75) | 0.02 (0.00–076) | 0.05 (0.00–0.81) | .758 | .779 | .841 |
| TNF-α | 0.27 (0.14–0.42) | 1.44 (0.58–2.65) | 12.64 (4.14–25.52) | 0.20 (0.08–0.30) | 0.93 (0.39–1.94) | 6.80 (1.69–15.00) | .289 | .140 | .140 |
| Intermediate monocytes | |||||||||
| IL1-α | 1.91 (0.55–4.39) | 2.54 (0.37–5.49) | 15.54 (3.30–26.96) | 0.18 (0.04–0.40) | 0.19 (0.01–0.61) | 1.86 (0.50–7.87) | <.001* | .001** | .086*** |
| IL1-β | 1.37 (0.57–3.85) | 9.29 (4.00–15.08) | 32.71 (16.84–46.76) | 0.56 (0.18–0.78) | 2.86 (1.48–8.70) | 10.56 (3.25–29.67) | .004** | .007** | .072*** |
| IL-6 | 1.28 (0.67–2.67) | 5.86 (3.69–11.42) | 24.55 (8.89–40.95) | 0.62 (0.22–1.61) | 4.45 (1.88–7.80) | 11.00 (4.76–21.62) | .021** | .211 | .192 |
| IL-8 | 4.42 (1.99–7.58) | 14.24 (10.94–24.72) | 36.35 (15.88–51.62) | 0.77 (0.43–3.86) | 10.83 (2.63–18.52) | 21.44 (7.18–37.25) | .003** | .049** | .157 |
| IL-10 | 2.10 (0.58–4.39) | 0.10 (0.00–1.69) | 0.14 (0.00–1.59) | 0.41 (0.06–1.56) | 0.05 (0.00–0.78) | 0.11 (0.00–1.64) | .004** | .738 | .904 |
| TNF-α | 0.57 (0.39–1.07) | 3.14 (1.33–5.54) | 13.02 (2.75–29.16) | 0.30 (0.12–0.61) | 0.89 (0.53–2.65) | 4.49 (1.94–14.00) | .026** | .030** | .183 |
| Nonclassical monocytes | |||||||||
| IL1-α | 1.10 (0.56–2.12) | 0.33 (0.00–2.00) | 0.48 (0.00–0.88) | 0.31 (0.15–0.55) | 0.35 (0.00–1.06) | 0.30 (0.00–1.55) | <.001* | .841 | .947 |
| IL1-β | 1.52 (0.75–3.90) | 1.73 (0.51–4.97) | 1.69 (0.00–5.00) | 0.50 (0.18–1.22) | 1.05 (0.50–3.43) | 1.10 (0.16–1.93) | .001** | .565 | .738 |
| IL-6 | 0.87 (0.23–1.83) | 0.81 (0.08–2.58) | 0.92 (0.11–3.03) | 0.23 (0.08–0.83) | 0.31 (0.00–1.24) | 0.16 (0.02–1.62) | .068*** | .341 | .192 |
| IL-8 | 6.81 (2.35–11.78) | 1.19 (0.00–4.21) | 1.70 (0.16–6.35) | 1.45 (0.70–6.67) | 1.23 (0.37–4.29) | 3.45 (0.32–6.38) | .038** | .583 | .565 |
| IL-10 | 4.21 (1.05–9.97) | 0.77 (0.00–2.08) | 0.00 (0.00–1.63) | 2.37 (1.04–5.16) | 0.40 (0.14–2.24) | 0.60 (0.00–1.96) | .327 | .678 | .211 |
| TNF-α | 2.43 (1.14–3.91) | 1.67 (0.23–3.31) | 1.56 (0.18–3.60) | 1.12 (0.54–1.63) | 1.17 (0.32–4.78) | 1.03 (0.22–4.04) | .010** | .883 | .925 |
Notes: Values are expressed as the median and interquartile range. Mann–Whitney U test was used to compare the values of the elderly group with those of the the young control group.
*p < .001. **p < .05. ***p < .1.