Figure 2. Schematic of RNA sequencing and follow up qPCR and population genetic work.

RNA from embryos of the AC and SB charr at four stages (AC embryos pictured at top) were sequenced with Illumina technology. To verify differentially expressed genes we used RNA from embryos and heads of these four morphs, and tissues from adult AC charr. To verify SNPs we genotyped population samples from three Lake Thingvallavatn morphs (PL, LB and SB).