Table 3. Correspondence of transcriptome and qPCR verification on Arctic charr embryos.
Tissue | Name | Abbr | FDRm | FRDt | Effect | qPCR | Morph |
---|---|---|---|---|---|---|---|
Embryo | Alkaline phosphatase | Alp | 0.070 | 0.001 | 0.986 | * | SB |
Embryo | Chondroitin sulfate
N-acetylgalactosaminyltransferase 2 |
Cgat | 0.004 | 0.331 | -2.556 | ||
Embryo | Cytochrome c oxidase subunit 6B1 | Cox6b1 | 0.058 | 0.632 | -1.208 | ||
Embryo | B5X596 Keratin-associated protein 4-3 | Krtap4-3 | 0.012 | 0.278 | -1.986 | * | AC |
Embryo | Lysozyme C II | Lyz2 | 0.041 | 0.001 | 1.138 | * | SB |
Embryo | Natterin-like protein | Nattl | 0.000 | 0.000 | 2.755 | * | SB |
Embryo | NADH dehydrogenase [ubiquinone] 1
beta subcomplex subunit 6 |
Ndub6 | 0.098 | 0.670 | -1.175 | ||
Embryo | Poly [ADP-ribose] polymerase 6 | Parp6 | 0.108 | 0.379 | -0.986 | * | AC |
Embryo | Ubiquitin-like protein 5 | Ubl5 | 0.059 | 0.003 | -1.234 | ||
Head | Claudin-4 | Cldn4 | 0.068 | 0.000 | 1.343 | * | SB/LB |
Head | Major vault protein | Mvp | 0.065 | 0.528 | 0.958 | * | SB/LB |
Head | Junction plakoglobin | Jup | 0.051 | 0.006 | 1.147 | * | SB/LB |
Head | Lipolysis-stimulated lipoprotein receptor | Lsr | 0.013 | 0.043 | 1.369 | * | SB/LB |
Head | TGF-beta receptor type-2 | Tgfbr2 | 0.065 | 0.013 | 1.728 | * | SB/LB |
Head | Vitamin D3 receptor A | Vdra | 0.053 | 0.052 | 1.312 | * | SB/LB |
Head | Retinoic acid receptor gamma-A | Rarg | 0.012 | 0.001 | 1.403 | ||
Head | Adseverin | Scin | 0.007 | 0.000 | 1.578 | * | SB/LB |
Tissue: which tissue was studied
Abbr: abbreviated paralog group or gene name
FRDm: FDR for comparison of SB and AC-charr in transcriptome
FDRt: FDR for comparison among developmental timepoints in transcriptome
Effect: logarithm of fold change between morphs, positive is higher in SB and negative higher in AC-charr in transcriptome (logFC.morph in supplemental dataset 1)
qPCR: results consistent with transcriptome (*), a blank cell reflects lack of correspondence
Morph: which morph(s) had higher expression in qPCR verification