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. 2016 Apr 25;4:136. Originally published 2015 Jun 1. [Version 2] doi: 10.12688/f1000research.6402.2

Table 3. Correspondence of transcriptome and qPCR verification on Arctic charr embryos.

Tissue Name Abbr FDRm FRDt Effect qPCR Morph
Embryo Alkaline phosphatase Alp 0.070 0.001 0.986 * SB
Embryo Chondroitin sulfate
N-acetylgalactosaminyltransferase 2
Cgat 0.004 0.331 -2.556
Embryo Cytochrome c oxidase subunit 6B1 Cox6b1 0.058 0.632 -1.208
Embryo B5X596 Keratin-associated protein 4-3 Krtap4-3 0.012 0.278 -1.986 * AC
Embryo Lysozyme C II Lyz2 0.041 0.001 1.138 * SB
Embryo Natterin-like protein Nattl 0.000 0.000 2.755 * SB
Embryo NADH dehydrogenase [ubiquinone] 1
beta subcomplex subunit 6
Ndub6 0.098 0.670 -1.175
Embryo Poly [ADP-ribose] polymerase 6 Parp6 0.108 0.379 -0.986 * AC
Embryo Ubiquitin-like protein 5 Ubl5 0.059 0.003 -1.234
Head Claudin-4 Cldn4 0.068 0.000 1.343 * SB/LB
Head Major vault protein Mvp 0.065 0.528 0.958 * SB/LB
Head Junction plakoglobin Jup 0.051 0.006 1.147 * SB/LB
Head Lipolysis-stimulated lipoprotein receptor Lsr 0.013 0.043 1.369 * SB/LB
Head TGF-beta receptor type-2 Tgfbr2 0.065 0.013 1.728 * SB/LB
Head Vitamin D3 receptor A Vdra 0.053 0.052 1.312 * SB/LB
Head Retinoic acid receptor gamma-A Rarg 0.012 0.001 1.403
Head Adseverin Scin 0.007 0.000 1.578 * SB/LB

Tissue: which tissue was studied

Abbr: abbreviated paralog group or gene name

FRDm: FDR for comparison of SB and AC-charr in transcriptome

FDRt: FDR for comparison among developmental timepoints in transcriptome

Effect: logarithm of fold change between morphs, positive is higher in SB and negative higher in AC-charr in transcriptome (logFC.morph in supplemental dataset 1)

qPCR: results consistent with transcriptome (*), a blank cell reflects lack of correspondence

Morph: which morph(s) had higher expression in qPCR verification