Supplemental Table S1 A. qPCR primers used in this study.
| Gene | Description | Primer Sequence (5’- 3’) | Product
Size (bp) |
PCR
Efficiency |
Melting
Temperature (°C) |
Exon
Boundary |
|---|---|---|---|---|---|---|
| Actb | Beta Cytoskeletal Actin | F-GAAGATCAAGATCATCGCCC
R-CAGACTCGTCGTACTCCTGCT |
122 | 1.95 | 80.5 ± 0.7 | Yes |
| Alp | Alkaline phosphatase | F-ACAGCATACCTCTGTGGGG
R-GGTGGCATGGTTCACACG |
177 | 1.90 | 85.12 ± 0.5 | Yes |
| Cldn4 | Claudin-4 | F-GTGCTGTGC CATCCCAAG
R-CACCACACAGGTCATCCACA |
100 | 1.98 | 80.4 ± 0.6 | Yes |
| Cgat2 | Chondroitin beta-1,4-N-
acetylgalactosaminyltransferase 2 |
F-GAGAGCCACTTTACTGAGGGG
R-GAATGGACGGAAAAGAGTAACG |
120 | 1.98 | 81.86 ± 0.3 | Yes |
| Cox6b1 | Cytochrome c oxidase subunit VIb
isoform 1 |
F-GAGGGTCTACAAATCACTGTGC
R-CCTGGAGTCCTACTCATACAAACAT |
147 | 1.93 | 82.22 ± 0.7 | Yes |
| Ef1α | Eukaryotic Translation Elongation
Factor 1 Alpha |
F-GAAGATCGGCTATAACCCTGC
R-ACCTTCCATCCCTTGAACC |
111 | 1.94 | 81.36 ± 0.4 | Yes |
| If5a1 | Eukaryotic Translation Initiation
Factor 5A |
F-GGCTTCGTGGTGCTGAAG
R-CCATGTGGACCTTAGCGTG |
91 | 1.91 | 80.76 ± 0.6 | Yes |
| Jup | Junction plakoglobin | F-CACAGCAGACATACCAGGATG G
R-CTGGCGATCTCTCCCCTGTT |
109 | 1.97 | 81.0 ± 0.3 | Yes |
| Krtap4–3 | Keratin-associated protein 4–3 | F-GCGGGACATCTACACTGCTTA
R-AGAAGGCTAAAGTCTTAGTGACTATC |
151 | 1.89 | 81.88 ± 0.6 | Yes |
| Lsr | Lipolysis-stimulated lipoprotein
receptor |
F-TGCTGTCACTCTGGGCGA
R-CCGTCTGGGCAAGGTTCA G |
80 | 1.91 | 80.77 ± 0.5 | Yes |
| Lyz | Lysozyme | F-TTCCAGATCAACAGCCGCTA
R-GATCGCCACTGTGATGTCAT |
111 | 1.94 | 81.87 ± 0.7 | Yes |
| Mvp | Major vault protein | F-ACCAACTCCCAGGAGGCT
R-CCTCTCCAGACGACCACG |
75 | 1.97 | 78.93 ± 0.3 | Yes |
| Nattl | Natterin-like protein | F-GTGAAAGTCACCTGCATGAATG
R-CATCTCTCCTTTGTGGATACCC |
104 | 1.98 | 78.81 ± 0.8 | No |
| Nattl-1 | Natterin-like protein paralog-1 | F-AATCCGTGTCCTACCACAATGA
R-GGTGTGTCGGTCAAAGCA |
135 | 1.77 | 78.03 ± 0.1 | No |
| Nattl-2 | Natterin-like protein paralog-1 | F-TGAAATVTVTGTCTCATCACAAC
R-GGATCTGGTCGAGGTGGC |
163 | 1.72 | 80.50 ± 0.2 | No |
| Nattl-3 | Natterin-like protein paralog-1 | F-GTGACATCCGTTTCTCACCAG
R-GATGTGTCGGTCAAAGCG |
138 | 1.77 | 79.12 ± 0.2 | No |
| Ndub6 | NADH dehydrogenase 1 beta
subcomplex subunit 6 |
F-TGGTGGAGTGTTCGCCTT
R-CTCTCTGGGAGGTCTGGAA |
171 | 1.89 | 82.40 ± 0.3 | Yes |
| Parp6 | Poly (ADP-Ribose) Polymerase
Family, Member 6 |
F-CCGTATGAATACCGTTCCACAGG
R-CACCCAGATGTTGCCGTGCTT |
147 | 1.93 | 81.87 ± 0.7 | Yes |