Supplemental Table S2 A. Verification of candidate polymorphisms.
Primer sequences, melting temperatures and primary data.
| Sequence | Position | Forward primer | Reverse primer | Tm
forward |
Tm
reverse |
Paralogs |
|---|---|---|---|---|---|---|
| NC_000861.1 | 1829 | GTGCCTCAGACCCACCTAGA | TCTGTCGCCCGTACTAAGGT | 60.26 | 59.76 | No |
| NC_000861.1 | 3119 | GGCCAGAGTAAACACCGAGA | CCTGGATTACTCCGGTCTGA | 60.25 | 60.07 | No |
| NC_000861.1 | 3411 | GGCCAGAGTAAACACCGAGA | CCTGGATTACTCCGGTCTGA | 60.25 | 60.07 | No |
| NC_000861.1 | 8876 | GACGTCCTTCACTCCTGAGC | GGGCTCATAAACTGGTCGAA | 59.99 | 60.07 | No |
| NC_000861.1 | 15240 | ACCCTAAAACCGAACGATCC | TGGCTAGGAAGAGTCCGGTA | 60.19 | 59.83 | No |
| SS2U034121 | 233 | CTCAACGTGCTTGACCAGTG | CCCTTACCCTCCAGGATCTC | 60.5 | 59.89 | Yes |
| SS2U054644 | 1037 | AAGGACGGCCACTATGGTCT | GGGGCATAGAGTGCACAGG | 60.9 | 61.65 | Yes |
| SS2U054644 | 1188 | TCAGAGATAGTGAAGAAGATGCTG | CGTACTTGATAAGACCTGTCGGTA | 57.92 | 59.62 | No |
| SS2U054644 | 1283 | TCAGAGATAGTGAAGAAGATGCTG | CGTACTTGATAAGACCTGTCGGTA | 57.92 | 59.62 | No |
| SS2U055283 | 1822 | TGTGTGAGGTGGTTGAGGAG | GGGTCATTGCTCCCTACAGA | 59.7 | 60.07 | No |
| SS2U055923 | 615 | GTGGACCCAGAGGATGAGAA | AGAACCTGCTCCCAGTTTGA | 60.05 | 59.84 | No |
| SS2U058906 | 350 | GCCAAAACCTCCACAATGAT | AACTGGCCTTCCAGATCAGA | 59.8 | 59.8 | Yes/No |
Paralogs: indicates whether the PCR and sequencing yielded mixed products, indicative of paralogous genes.