FIG 3.

The comparison of Michaelis-Menten kinetics of AnoA and AioBA for As(III) and Sb(III), and the influence of H₂O₂ concentration on bacterial Sb(III) oxidation efficiency of A. tumefaciens GW4. (A and B) Kinetic data for As(III). (C and D) Kinetic data for Sb(III). The data from panel A are from our unpublished data, the data from panels B and D are from reference 123, and the data from panel C are from reference 117. (E) The growth curves of strain GW4, the katA mutant strain, and the katA complementary strain. (F) Sb(III) oxidation profiles of the three strains as in panel E (shown with the same symbols in panel E). (G and H) The lacZ reporter assays of katA gene with the addition of H₂O₂ and Sb(III), respectively (panels G and H have the same symbols). The data are shown as the mean of the results from three replicates, with the error bars representing the standard deviation (SD). v, volume; OD₆₀₀, optical density at 600 nm; d, days; β-gal, β-galactosidase; MU, Miller units.