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Effect of exogenously added DCSA or 5-methyl-THF on the conversion of dicamba. The reaction mixtures contained 10 mM Tris HCl (pH 8.0), 0.1 μg of purified Dmt, 1.0 mM dicamba, 2.0 mM THF, and various concentrations (0, 0.1, 0.3, 0.5, 0.7, and 1.0 mM) of 5-methyl-THF or DCSA in a final volume of 300 μl and were incubated for 120 min at 30°C.
