Skip to main content
. 2016 Aug 26;36(18):2418–2430. doi: 10.1128/MCB.00202-16

FIG 1.

FIG 1

MISR2 lineage tracing in ovarian and oviduct epithelia in wild-type and tubular adenomas of Wv/Wv mice. ROSA26 mice were crossed with Wv mutant and MISR2-Cre transgenic mice. At 3 to 4 months of age, the obtained progeny, including Wv/Wv; ROSA26; MISR2-Cre and ROSA26; MISR2-Cre or Wv/+; ROSA26; MISR2-Cre (as a control) genotypes, were analyzed for LacZ expression as a reporter of MISR2 lineage. More than 5 mice from each genotype were analyzed, and representative results are shown. (A) Typical example of LacZ staining of an ovary from a ROSA26; MISR2-Cre mouse. (B) Higher magnification of the ovary in panel A with both LacZ-positive (arrow) and -negative (arrowhead) ovarian surface epithelial cells. (C) Higher magnification of the ovary in panel A. Epithelial cysts located in the interior of the ovary were often LacZ positive, as shown in the examples (arrow). (D) Typical example showing oviduct fimbria of a ROSA26; MISR2-Cre mouse, where the stromal cells were LacZ positive and the epithelial cells were largely negative (arrowhead). (E) Rare LacZ-positive fallopian tube fimbrial epithelial cells (arrow) among largely LacZ-negative cells (arrowhead) in a ROSA26; MISR2-Cre mouse. (F) Typical example showing LacZ staining of uterine horns of a ROSA26; MISR2-Cre mouse, where the stroma was LacZ positive and the epithelial cells were largely negative (arrowhead). (G) Typical example of LacZ staining of an ovary and associated uterine horn and oviduct from a 3-month-old Wv/Wv; ROSA26; MISR2-Cre mouse. (H) Higher magnification of the ovary in panel G. Tubular adenomas located near the ovarian surface were largely LacZ positive (arrow) and mixed with some LacZ-negative cells (arrowhead). (I) Higher magnification of the ovary in panel G. Epithelial cysts and tubular adenomas located in the interior of the ovary were often LacZ positive (arrow). An example showing LacZ-negative cells is indicated by the arrowhead. (J) Based on the analysis of at the least 5 cases in each category, the percentages of LacZ-positive epithelial cells located on the surface or in the interior were estimated for control (ROSA26; MISR2-Cre or Wv/+; ROSA26; MISR2-Cre) and Wv/Wv (Wv/Wv; ROSA26; MISR2-Cre) mice.