FIG 7.

Increased proliferation of p27^−/− and p53^−/− mouse ovarian surface epithelial cells in culture. Primary ovarian surface epithelial cells were prepared from ovaries of mice with various genotypes. The growth rates of wild-type, Wv/Wv (early [passage 2] and late [passage 7] passages), p27^+/−, p27^−/−, p53^+/−, and p53^−/− primary ovarian surface epithelial cells were measured by WST-1 assay (n = 3) from days 1 to 5. The results are shown as means ± standard deviations (SD). The differences were found to be statistically significant between p27^+/− or p27^+/− and wild-type and between p53^−/− and wild-type or p53^+/− cells.