FIG 8.

Analysis of markers in p27^+/−, p27^−/−, and p53^−/− mouse ovarian surface epithelial cells. Primary ovarian surface epithelial cells were prepared from ovaries of 3-month-old mice with wild-type, p27^+/−, p27^−/−, p53^+/−, and p53^−/− genotypes. Early (passage 3) and late (passage 8) p53^−/− cells were used. The cells were analyzed for various markers by Western blotting. (A) Cell cycle and proliferation markers were analyzed in wild-type (wt), p27^+/−, and p27^−/− cells. (B) Cell cycle and proliferation markers were analyzed in wild-type and early (passage 3 [p.3]) and late (passage 8) passages of p53^−/− cells. (C and D) Cyclin inhibitors. (E and F) Epithelial-to-mesenchymal transition markers. (G and H) Markers of cellular signaling. (I and J) Apoptosis and cell survival markers. The aligned panels of images from the immunoblots were derived from the same cell lysate, but more than one gel was used to obtain all the images. Thus, the beta-actin loading controls are representative but not necessarily from the same gel.