FIG 10.
Reciprocal regulation of 2-hydroxyglutarate production by 25-hydroxycholesterol or statins in IDH1R132C-mutant cells. (A) Normalized peak areas of 13C-labeled (M+2) or unlabeled (M+0) metabolites measured by LC-MS/MS in metabolite extracts from HT1080 cells cultured for 48 h in the presence of 25-HC (1 μg/ml) or atorvastatin (5 μM) and pulse-labeled with [U-13C]glucose for 20 min. The data are presented as the means ± the SEM of biological triplicates and are representative of two independent experiments (*, P < 0.05 relative to untreated cells). (B) Model of SREBP-mediated control of wild-type and oncogenic IDH1, their metabolic functions, and de novo lipid synthesis.