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. 2016 Aug 15;5(8):e253. doi: 10.1038/oncsis.2016.47

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Overexpression of RCAN2 in RKO and SW837 human colorectal cancer cell lines. RKO (BRAF-mutated) and SW837 (KRAS-mutated) cells showing weak expression of RCAN2 were infected with RCAN2/pDON-5 and pDON-5/empty retroviruses. RCAN2 expression (a) and calcineurin activity (b) were compared between cells infected with RCAN2/pDON-5 and pDON-5/empty (black and white bars, respectively). Proliferation and migration were compared in RKO cells with or without RCAN2 overexpression (c, d, respectively) and SW837 cells with or without RCAN2 overexpression (e, f, respectively).