Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 15;5(8):e253. doi: 10.1038/oncsis.2016.47

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

shRNA knockdown of RCAN2 in the Colo320 human colorectal cancer cell line. RCAN2 expression in Colo320 cells (both KRAS and wild-type BRAF) with high expression of RCAN2 was knocked down using an shRNA vector. RCAN2 expression (a) and calcineurin activity (b) were compared between cells infected with pSUPER/RCAN2 1, 2 and pSUPER/non-silencing. These cells were subjected to proliferation and wound-scratch migration assays (c, d, respectively). In vivo tumorigenesis was compared between Colo320 cells infected with pSUPER/RCAN2 1 and pSUPER/non-silencing using a xenograft subcutaneous transplantation model in order to determine tumor volume (e) and tumor weight (f). Images of harvested tumors are shown in g.