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. 2016 Aug 15;5(8):e253. doi: 10.1038/oncsis.2016.47

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Proliferation assay in Colo320 cells, with or without RCAN2 shRNA knockdown, following calcineurin inhibitor treatment. Colo320 cells infected with pSUPER/RCAN2 shRNA1 and pSUPER/non-silencing shRNA were cultured in medium with or without either cyclosporine A or tacrolimus, and cell proliferation (a, c, respectively) and enzyme activity (b, d, respectively) were subsequently analyzed. Black line: pSUPER/RCAN2 shRNA1 without treatment; gray line: pSUPER/RCAN2 shRNA1 with calcineurin inhibitors; black dashed line: pSUPER/non-silencing shRNA without treatment; black dashed line: pSUPER/non-silencing shRNA with calcineurin inhibitors. CsA, cyclosporine A; FK506, tacrolimus.