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. 2016 Aug 8;5(8):e250. doi: 10.1038/oncsis.2016.52

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Evaluation of wt-p53 and microRNA-125b (miRNA-125b) transfection in SK-LU-1 lung adenocarcinoma cells and exosomes using plasmid DNA-encapsulated hyaluronic acid-poly(ethylene imine)/hyaluronic acid-poly(ethylene glycol) (HA-PEI/HA-PEG) nanoparticles. (a) Quantitative qRT–PCR analysis of expression of wt-p53 in cells (p53/cells) and in exosomes (p53/exo) when transfected with wt-p53 expressing plasmid DNA alone or in combination with miRNA-125b expressing plasmid DNA in cells (combi/cells) and in exosomes (combi/exo) after 18 h of incubation. (b) Qualitative and quantitative analysis of Flag-p53 protein expression using western blot in cells (p53/cells) and in exosomes (p53/exo) when transfected with wt-p53 expressing plasmid DNA alone or in combination with miRNA-125b expressing plasmid DNA in cells (combi/cells) and in exosomes (combi/exo) after 18 h of incubation. (c) Quantitative qRT–PCR analysis of expression of miR-125b expression in cells (miR-125b/cells) and in exosomes (miR-125b/exo) when transfected with miRNA-125b expressing plasmid DNA alone or in combination with wt-p53 expressing plasmid DNA in cells (combi/cells) and in exosomes (combi/exo) after 18 h of incubation. (*) P<0.05. Data represents mean±s.e.m., n=6.