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. 2016 Aug 8;5(8):e250. doi: 10.1038/oncsis.2016.52

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Macrophage repolarization with exosomes from wt-p53 and miRNA-125b transfected SK-LU-1 cells. (a) qRT–PCR analysis following exosome-induced changes in the miR-125b expression profile of J774.A1 murine macrophages. The J774.A1 cells pre-polarized to M2 phenotype using IL-4 were treated with exosomes from non-transfected SK-LU-1 cells (SK/exo) and exosomes obtained after transfection with plasmid DNA expression wt-p53 (p53/exo), miRNA-125b (miR-125b/exo) and combination wt-p53/miR-125b (combi/exo)-transfected SK-LU-1 cells after 18 h. (b) Schematic of the experimental design for exosome-mediated changes in the iNOS/Arg-1 gene expression ratio as well as pro- (tumor necrosis factor (TNF)-α and IL-1β) and anti-inflammatory (IL-10) cytokine expression in J774.A1 macrophages after treatment with exosomes from non-transfected and transfected SK-LU-1 cells. (c) The expression ratio of iNOS/Arg1 mRNA in J774.A1 macrophages after treatment with exosomes from non-transfected SK-LU-1 cells (SK/exo) and exosomes obtained after transfection with plasmid DNA expression wt-p53 (p53/exo), miRNA-125b (miR-125b/exo) and combination wt-p53/miR-125b (combi/exo)-transfected SK-LU-1 cells after 18 h. (d) The expression of pro- (TNF-α and IL-1β) and anti-inflammatory (IL-10) cytokine expression in J774.A1 macrophages after treatment with exosomes from non-transfected SK-LU-1 cells (SK/exo) and exosomes obtained after transfection with plasmid DNA expression wt-p53 (p53/exo), miRNA-125b (miR-125b/exo) and combination wt-p53/miR-125b (combi/exo)-transfected SK-LU-1 cells after 18 h. For qRT–PCR analysis, all of the individual gene expression markers were normalized to beta-actin and untreated control samples using relative quantification by delta(delta(Ct) method. * indicates comparison against control designated as 1. One-way ANOVA followed by post hoc t-test with multiple comparisons, *P<0.05. Data represent as mean±s.e.m., n=6.