Table 3. MicroRNA-associated functional pathways in 125b/exo.
| # | KEGG pathway | # of genes | # of microRNAs | P-value |
|---|---|---|---|---|
| 1 | Focal adhesion (hsa04510) | 119 | 55 | 1.48E-17 |
| 2 | PI3K–Akt signaling pathway (hsa04151) | 189 | 54 | 2.60E-14 |
| 3 | MAPK signaling pathway (hsa04010) | 135 | 50 | 4.09E-11 |
| 4 | mTOR signaling pathway (hsa04150) | 40 | 47 | 1.05E-08 |
| 5 | Ubiquitin mediated proteolysis (hsa04120) | 80 | 44 | 4.76E-10 |
| 6 | p53 signaling pathway (hsa04115) | 43 | 40 | 2.10E-08 |
| Calcium signaling pathway (hsa04020) | 48 | 40 | 2.30E-03 | |
| 7 | ErbB signaling pathway (hsa04012) | 55 | 39 | 3.43E-10 |
| 8 | HTLV-1 infection (hsa05166) | 84 | 38 | 5.09E-05 |
| 9 | Pathways in cancer (hsa05200) | 172 | 36 | 2.91E-08 |
| 10 | Wnt signaling pathway(hsa04310) | 92 | 36 | 5.17E-07 |
| 11 | Melanoma (hsa05218) | 44 | 36 | 1.54E-07 |
| 12 | Transcriptional misregulation in cancer (hsa05202) | 93 | 34 | 1.22E-05 |
| 13 | Colorectal cancer (hsa05210) | 37 | 34 | 4.21E-05 |
| 14 | Small cell lung cancer (hsa05222) | 49 | 32 | 2.74E-13 |
| 15 | TGF-beta signaling pathway (hsa04350) | 57 | 31 | 2.65E-12 |
| 16 | Renal cell carcinoma (hsa05211) | 43 | 30 | 4.07E-09 |
| 17 | ECM–receptor interaction (hsa04512) | 35 | 26 | 4.82E-113 |
| 18 | Glycosaminoglycan biosynthesis—chondroitin sulfate (hsa00532) | 9 | 15 | 4.43E-10 |
We collected the exosomes secreted from SK-LU-1 cells transfected with HA nanoparticles encapsulated with miR-125b plasmid. miRNA expression was undertaken using Nanostring. Using microT-CDs target predicting data set along with mirPATH software, we characterized the top KEGG pathways predicted for interaction enrichments for miRNAs in 125b/exo. Ln(P-value) is a natural logarithmic scale of the P-value obtained from a χ2 test comparing expected number of genes with interaction and the actual number composing the pathways (DIANA algorithm). The third column indicates the number of genes identified as targets of the differentially expressed miRNAs in the specific groups enlisted in the fourth column. Abbreviations: ECM, extracellular matrix; HTLV-1, human T-lymphotrophic virus-1.