Table 4. miRNA-associated functional pathways in combi/exo.
| # | KEGG pathway | # of genes | # of microRNAs | P-value |
|---|---|---|---|---|
| 1 | ECM–receptor interaction (hsa04512) | 38 | 18 | 4.82E-113 |
| 2 | Pathways in cancer (hsa05200) | 207 | 34 | 1.35E-17 |
| 3 | Focal adhesion (hsa04510) | 128 | 34 | 1.48E-17 |
| 4 | Transcriptional misregulation in cancer (hsa05202) | 109 | 27 | 4.47E-16 |
| 5 | Ubiquitin mediated proteolysis (hsa04120) | 90 | 32 | 1.98E-14 |
| 6 | PI3K–Akt signaling pathway (hsa04151) | 208 | 35 | 2.60E-14 |
| 7 | Small cell lung cancer (hsa05222) | 50 | 23 | 2.74E-13 |
| 8 | Endocytosis (hsa04144) | 117 | 22 | 3.80E-13 |
| 9 | ErbB signaling pathway (hsa04012) | 63 | 34 | 5.51E-12 |
| 10 | MAPK signaling pathway (hsa04010) | 164 | 37 | 4.09E-11 |
| 11 | p53 signaling pathway (hsa04115) | 49 | 32 | 2.72E-09 |
| 12 | Regulation of actin cytoskeleton (hsa04810) | 124 | 28 | 1.83E-08 |
| 13 | Cytokine–cytokine receptor interaction (hsa04060) | 54 | 12 | 6.12E-07 |
| 14 | Fc gamma R-mediated phagocytosis (hsa04666) | 52 | 18 | 7.97E-07 |
| 15 | NF-kappa B signaling pathway (hsa04064) | 12 | 4 | 1.14E-06 |
| 16 | T-cell receptor signaling pathway (hsa04660) | 50 | 12 | 2.52E-06 |
| 17 | HIF-1 signaling pathway (hsa04066) | 59 | 15 | 1.75E-05 |
| 18 | HTLV-I infection (hsa05166) | 135 | 30 | 0.000172889 |
| 19 | Notch signaling pathway (hsa04330) | 30 | 12 | 0.000292382 |
| 20 | Toll-like receptor signaling pathway (hsa04620) | 23 | 5 | 0.000409522 |
| 21 | VEGF signaling pathway (hsa04370) | 30 | 10 | 0.000431036 |
| 22 | Chemokine signaling pathway (hsa04062) | 72 | 10 | 0.000437235 |
| 23 | Cell adhesion molecules (CAMs) (hsa04514) | 34 | 8 | 0.000764716 |
| 24 | Natural killer cell mediated cytotoxicity (hsa04650) | 21 | 3 | 0.001346897 |
| 25 | Apoptosis (hsa04210) | 32 | 7 | 0.008855041 |
We collected the exosomes secreted from SK-LU-1 cells transfected with HA nanoparticles encapsulated with wt-p53 plasmid in combination with HA nanoparticles encapsulating miR-125b plasmid. miRNA expression was undertaken using Nanostring. Using microT-CDs target predicting data set along with mirPATH software, we characterized the top KEGG pathways predicted for interaction enrichments for miRNAs in combi/exo. Ln(P-value) is a natural logarithmic scale of the P-value obtained from a χ2 test comparing expected number of genes with interaction and the actual number composing the pathways (DIANA algorithm). The third column indicates the number of genes identified as targets of the differentially expressed miRNAs in the specific groups enlisted in the fourth column.Abbreviations: ECM, extracellular matrix; HIF-1, hypoxia-inducible factor-1; HTLV-1, human T-lymphotrophic virus-1; VEGF, vascular endothelial growth factor.