Figure 1.

RAG1^−/− Sgo1^−/+ mice developed lung adenocarcinomas. (a) RAG1^−/− Sgo1^−/+ mice developed lung adenocarcinomas with the appearance of bronchioloalveolar in-type (that is, cuboidal cells lining the alveolar septa and projecting into alveolar spaces). RAG1^−/− and most Sgo1^−/+ did not show significant histopathological changes, with some exceptions in Sgo1^−/+ that developed adenocarcinomas in smaller sizes. (b) To assess DNA double-strand break, IHC for γ-H2AX was performed. Percentages for γ-H2AX positive cells were high in Branchioli, but varied between Sgo1^−/+ and Sgo1^+/+ backgrounds in alveoli. Al, alveole; Br, branchiole. (c) Rag1^−/−Sgo1^−/+ showed highest percentage of γ-H2AX positive cells. However, signals at branchioli showed little difference (NS, nonsignificant). The difference between Sgo1^−/+ and Sgo1^+/+ backgrounds were evident only in alveoli.