Figure 3.

Analysis of cell lines with overexpression and knockdown of RBP2. (a) Stable βlox5 cells overexpressing RBP2, mRBP2 or empty vector were assessed for proliferation every day for 4 days. Similar proliferation assays were performed every other day for days for the H727 and QGP-1 cells lines that were transiently transfected with scrambled si or RBP2si, with the assay beginning 24 h after transfection. (b) mRNA levels of p21, p27 and p57 in stable overexpressing βlox5 cells, and H727 and QGP-1 transiently transfected with RBP2 siRNAs. (c) Representative western blots for the same targets in cell lines as above in (b) and protein quantitation via densitometry of p21, p27 and p57 resulting from three separate experiments using actin for normalization. Significance was measured relative to the respective control (*P<0.05 **P<0.02 ^#P<0.005).