Figure 5.

β-Nitrostyrenes (9, 12, 14, and 18) induced nitric oxide production in ex vivo model. Macrophages isolated from peritoneal cavity of BALB/c mice, infected with promastigotes and stimulated with β-nitrostyrenes (9, 12, 14, and 18) compounds at 37°C in a CO₂ incubator with serial four-fold dilutions of test compounds (starting at 25–0 μM) for 48 h. Nitrite generation was determined by Gries methods, as described in Methods. Each point corresponds to the mean ± SE of triplicate samples and is representative of one of three independent experiments.