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. 2016 Jun 30;5(8):e1199311. doi: 10.1080/2162402X.2016.1199311

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 7. — CIK cell-mediated ADCC is independent of TCRγ/δ⁺ subpopulation. (A) Flow cytometry analysis was performed in quadruple fluorescence to determine the expression of CD16 in TCRα/β (n = 8) and TCRγ/δ (n = 14) subpopulations of CD3⁺CD56⁺ CIK cells from different donors. (B) TCRγ/δ⁺ cells were removed by magnetic beads depletion. The panels show one representative experiment out of 5 performed, in which flow cytometry analysis was carried out before (left) and after (right) depletion. (C) CIK cells were tested for cytotoxicity against SKOV-3 and MDA-MB-468 tumor cell lines. Lytic activity was measured using bulk CIK cells (circles) or TCRγ/δ-depleted CIK cells (triangles), in the absence (open symbols) or presence (black symbols) of the corresponding mAb (trastuzumab for SKOV-3, and cetuximab for MDA-MB-468). The symbols refer to the specific lysis of individual CIK cell cultures from different donors at an E/T ratio of 50:1, and bars indicate mean values ± SD. Data were analyzed by Student's t-test (+++, p ≤ 0.001; ++, p ≤ 0.01).