Fig. 5.

IgLON5 antibodies irreversible decrease IgLON5 clusters on cell surface. a Immunofluorescence on hippocampal neurons treated for 7 days (14DIV to 21DIV) with control or patient with anti-IgLON5 IgG. The surface clusters are drastically reduced by the anti-IgLON5 IgG, and the effect is irreversible because 7 days after removing the anti-IgLON5 IgG the reduced number of surface IgLON5 clusters persisted. Scale bar = 5 μm. b Quantification of the decrease of IgLON5 clusters on the dendrite surface in a time-course treatment of 1, 3, and 7 days, and 7 days after removing the anti-IgLON5 IgG. ***p < 0.005, ****p < 0.0001. c Synaptic markers (PSD95, synapsin-I) were not affected by the anti-IgLON5 IgG treatment