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. 2016 Sep 1;16(1):190. doi: 10.1186/s12870-016-0880-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Selection of hydrolysis time for seeds. LC-ESI-MS-QTOF(AutoMSMS) analysis of non-hydrolysed Col-0 seed samples (n = 10). a Merged EICs for quercetin-bases glycosylated flavonoids with the strongest response. b Extracted non-hydrolysed samples before (-) and after addition of acid (+) and heat treatments. c non-extracted samples after addition of acid (+) and heat treatments. *: extracted non-hydrolysed samples are shown for comparison. acid: HCl in MeOH. Boxed: selected hydrolysis time derived from this experiment. See Additional file 4: Figure S6 and Table S10