FIG 5.
Comparison of viral replication in brains infected with CE(NiP)- and CE(NiP)ΔP2-5-Luc in vivo. (A) Brains of mice inoculated with 104 FFU of CE(NiP)-Luc and CE(NiP)ΔP2-5-Luc via the i.c. route were collected at 1, 3, and 5 dpi. Luciferase activities in the brains were measured and calculated as relative light units (log10 RLU/s/g brain weight). (B) Propagation of CE(NiP) and CE(NiP)ΔP2-5 in adult mouse brains. Mice were inoculated with 104 FFU of each virus via the i.c. route. Viral titers in brains collected at 5 dpi were determined by focus assays (FFU/g brain weight).