FIG 7.

Comparison of viral replication in muscle cells infected with CE(NiP)- and CE(NiP)ΔP2-5-Luc in vivo and in vitro. (A) Thigh muscles of mice inoculated with 10⁶ FFU of CE(NiP)-Luc and CE(NiP)ΔP2-5-Luc via the i.m. route were collected at 0, 24, 48, and 72 hpi. Lysates of muscles were prepared and used to measure luciferase activities calculated as relative light units (RLU [×10³]/s/g muscle weight). (B) Muscle G-8 cells inoculated with CE(NiP)-Luc and CE(NiP)ΔP2-5-Luc at an MOI of 1 were collected at 1, 3, and 5 dpi. Cell lysates were prepared and used to measure luciferase activities, calculated as relative light units (RLU [×10²]/s). (C) Growth curves of CE(NiP) and CE(NiP)ΔP2-5 in G-8 cells. Each virus was inoculated into G-8 cells at an MOI of 1. Viral titers in culture supernatants collected at 1, 3, and 5 dpi were determined by focus assays. All assays were carried out in triplicate, and the values in the graph are shown as means ± standard errors of the means. *, Significant difference at a P value of <0.05; **, significant difference at a P value of <0.01.