FIG 1.

Survival and viral burden analysis of WT and Ifitm3^−/− mice. (A) Eight- to 10-week-old age-matched WT (n = 28) and Ifitm3^−/− (n = 23) C57BL/6 mice were infected via the subcutaneous route with 10² FFU of WNV (New York 1999) and monitored for mortality for 21 days. Survival differences were analyzed by the log rank test (P < 0.005). (B to F) WNV tissue burden in WT and Ifitm3^−/− mice after subcutaneous infection. WNV levels in serum (B), draining lymph node (C), spleen (D), brain (E), and spinal cord (F) were measured by qRT-PCR (B and C) or plaque assay (D to F). Solid lines represent the median viral titers, and dotted lines denote the limit of detection of the assay. Two or three mice were used per independent experiment,and 5 to 7 independent experiments were performed. Asterisks indicate statistically significant differences by the Mann-Whitney test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).