FIG 3.
Heat-inactivated KyA (HI-KyA) does not protect CBA mice from RacL11 challenge. (A) Schematic representation of HI-KyA immunization and RacL11 challenge infection of CBA mice. CBA mice (n = 8) were immunized with medium (control), KyA (2 × 106 PFU/mouse), or HI-KyA (equivalent to 2 × 106 PFU prior to inactivation/mouse) at day 0 and day 14 and were challenged with RacL11 (1.5 × 106 PFU/mouse) at 28 days after the first immunization. (B and C) Average body weights (B) and percentages of survival (C) of CBA mice challenged with RacL11 at 28 days postimmunization. D, twice immunized.