FIG 6.

Validation of Affymetrix microarray data by real-time PCR analysis. The relative mRNA expression of seven of the most highly upregulated ISGs and the IFN-γ gene, shown in Table 3, was quantified using qRT-PCR. Data were normalized to GAPDH expression. The genes selected are seven antiviral ISGs (RSAD2, MX1, IRF7, IFI44L, ISG15, OAS1a, and IFI204) and the IFN-γ gene. Data for KyA and RacL11 were obtained at 8 h postinfection. Data for KyA- or heat-inactivated KyA-immunized mice challenged with RacL11 (KyA → RacL11 or HI-KyA → RacL11, respectively) were obtained at 8 h post-RacL11 challenge. P values for KyA → RacL11 and HI-KyA → RacL11 mice are shown in Table 4. Results are expressed as the fold change from expression in the mock-infected control.